Table 3.

Dissociation (k _off) rate constants of [³H]muscimol binding at +4 °C in recombinant receptors expressed in HEK293 cells

Recombinant receptors	k off (min−1)
α1β2γ2	0.352 ± 0.009
α6β2γ2	0.086 ± 0.010*** , **
α6β2δ	0.015 ± 0.003***

Dissociation rate constants (k _off) of [³H]muscimol binding from recombinant receptors (n = 3 independent transfections and independent experiments performed in triplicate technical replicates. The results are expressed as mean ± SEM values). Statistical comparison of recombinant receptor values: ***p < 0.001, significantly different from the corresponding α1β2γ2 values; **p < 0.01, significantly different from the α6β2δ value (one‐way anova followed by Tukey's post hoc test).