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. Author manuscript; available in PMC: 2020 Apr 1.
Published in final edited form as: J Neurochem. 2019 Mar 15;149(1):98–110. doi: 10.1111/jnc.14668

Figure 1. Effects of Tat on OL survival.

Figure 1.

(A) Tat treatment led to a dose-dependent decrease of immature OL survival, but did not affect survival of mature OLs. In 4 independent experiments, ≥ 50 OLs were tracked for every treatment during the course of the entire 96 h time period; survival effects were analyzed by repeated measures two-way ANOVA. (B-C) Some results from (A) were confirmed by a fluorescent viability assay using Calcein-AM (green) and EthD-1 (red), and quantified with ImageJ software. Sample images in (C) were chosen from vehicle and Tat-treated immature OL groups assayed at 96 h. (* p < 0.05 vs. corresponding control group; # p < 0.05 vs. corresponding 100 nM Tat group; Scale bar: 10 μm; C: control; T: 100 nM Tat). For each experiment, N=4 independent cell cultures prepared from mice of different litters.