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. Author manuscript; available in PMC: 2020 Apr 1.
Published in final edited form as: Arthritis Rheumatol. 2019 Mar 6;71(4):583–593. doi: 10.1002/art.40751

Figure 2. ZCCHC6 regulates IL-6 expression in chondrocytes.

Figure 2.

(A) Cytokine multiplex assay and qPCR were performed with the culture supernatant of ZCCHC6 depleted human chondrocytes and Zcchc6−/− mouse chondrocytes and total RNA prepared from ZCCHC6 depleted human chondrocytes and their respective controls. The cytokine expression from all the three different experiments were compared and the Venn diagram showed that IL-6 was the common cytokine downregulated in both human and mouse chondrocytes. (Human plex = cytokine multiplex assay of human samples with a total number of cytokines=65 and number of affected cytokines was 40. Mouse plex = cytokine multiplex assay of mouse samples with a total number of cytokines = 36 and number of affected cytokines was 14. Human PCR = cytokine PCR array of human samples with a total number of targets = 88 and number of affected targets was 58). (B) siRNA mediated knockdown of ZCCHC6 at mRNA and protein level was confirmed by qPCR and Western blotting respectively. (C) Human OA chondrocytes with siRNA mediated knockdown of ZCCHC6 expressed low levels of constitutive and IL-1β induced compared to controls (*p<0.05). (D) Overexpression of ZCCHC6 in human OA chondrocytes induced the constitutive and IL-1β induced expression of IL-6 (*p<0.05).