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. Author manuscript; available in PMC: 2020 Apr 1.
Published in final edited form as: Arthritis Rheumatol. 2019 Mar 6;71(4):583–593. doi: 10.1002/art.40751

Figure 5: IL-6 mRNA expression is regulated by miR26a/b in chondrocytes.

Figure 5:

(A) Targetscan prediction showed a conserved site for miR26a/b family of miRNAs in the 3’-UTR of IL-6 mRNA. (B) The expression level of miR26a or miR26b was not altered in human chondrocytes with knockdown of ZCCHC6. (C) Expression of miR26a and miR26b suppressed the IL-1β induced expression of IL-6 in human OA chondrocytes (*p<0.05). (D) 3’-uridylation analyses of miR26a and miR26b by deep sequencing showed decreased uridylation at +1 and +2 position. (E) Human OA chondrocytes were transfected with miR26a or miR26b mimetics and miR26a and miR26b mimetics with additional mono- or di-Uridines at the 3’-ends followed by IL-1β treatment for 6 hrs and total RNA was prepared for qPCR analysis of IL-6 mRNA. miR26b mimetics with di-uridine at the 3’-end failed to suppress the IL-1β-induced expression of IL-6 mRNA (*p<0.05). (–)ve control represent scrambled oligo. The experiments were repeated with chondrocytes from at least three OA donors.