Table 3.
Precise IC50 and volume/dose index values for pterostilbene showing strong inhibition of DEAQ, hydroxypioglitazone and serotonin glucuronide formation.
| Enzyme | IC50 (µM) | V/D index (L/dose) |
|---|---|---|
| CYP2C8 (Amodiaquine) | 3.0 ± 0.4 | >50 |
| CYP2C8 (Pioglitazone) | 18.0 ± 2.2 | >50 |
| UGT1A6 (Serotonin) | 15.1 ± 2.8 | >50 |
Amodiaquine, pioglitazone and serotonin and pooled HLM were incubated with different concentrations of pterostilbene as mentioned under materials and methods. All incubations were performed in duplicate and the data represent the best-fit IC50 values ± standard error. The r2 values for goodness of fit were >0.9. The recommended daily intake values (250 mg) were determined based on the commercially available products and a previous clinical study (Riche, 2012).