Figure 5. High expression of miRNA-654-5p promoted motility in breast cancer cells. (A) The basic expression of miRNA-654-5p in 3 different breast cancer cell lines. (B, C) The efficiency after transfection of miRNA-654-5p mimics in MDA-MB-231 cells. (D) Wound healing indicated that the miRNA-654-5p mimic group migrated significantly faster than the control group (×40). (E, F) The decreased area in the treatment group was apparently more than that in the negative control group after 24 hours (p < 0.05). (G, H) The number of cells migrated to the lower wells in the treatment group were more than the negative group (×40, ×100, p < 0.05).

cq = cycle quantification; NC = negative control.