Figure 6. Upregulated miRNA-654-5p changed the expression of the protein related to the metastasis verified by quantitative polymerase chain reaction and Western blotting. (A) For the expression in the negative control and the standard, the expression of the gene involved in migration was upregulated, especially the expression of snail (p < 0.05). (B) Cellular lysates from the experimental group cells were used to measure epithelial-mesenchymal transition-related protein expression by western blot. (C) F-actin staining (left panels) of MDA-MB-231 cells transfected with synthetic miRNA-654-5p for 4 days. DAPI staining was used to detect the nuclei. The merged panels showed different cellular morphology.

DAPI = 4′,6-diamidino-2-phenylindole; GAPDH = glyceraldehyde 3-phosphate dehydrogenase; NC = negative control.