Figure 5.

Treatment with GA inhibited the growth of A549 cells in vivo. (A) and (B) The GA treatment suppressed the formation of tumours by A549 cells in vivo. Mice were treated with 0.9% NaCl (model), GA (high dose, 100 mg/kg; low dose, 50 mg/kg) or Kobe (80 mg/kg). (A) Tumour weights were monitored. (B) Growth curves of the xenograft tumours. Values are presented as means ± S.D. (n = 6). (C) Images of H&E (original magnification × 100 and × 400), and IHC staining of the tumour tissues show the levels of P-ERK1/2, scale bar: 50 µm. (D) and (E) Levels of the phosphorylated c-RAF-S259, B-RAF-S729, B-RAF-T401 and ERK1/2 proteins in the tumour tissues were analysed by Western blotting. The data are reported as means ± S.D. (n = 3). ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 compared to the model.