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. 2019 Mar 22;10:570. doi: 10.3389/fmicb.2019.00570

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Copyright © 2019 Rojo-Molinero, Macià and Oliver.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Three-dimensional images and transversal sections of competition experiments (1:1 initial ratio) between ECFP-tagged (cyan) PAO1 and its EYFP-tagged (yellow) derivative PAOΔdacB and ECFP-tagged (cyan) single biofilm treated with FEP (0, 1, 2, or 4 mg/l). Images obtained at t0 and t6 (8-day-old biofilms, 6 days of FEP treatment) are shown. Red arrows mark mutant strain surrounding PAO1.