Skip to main content
. 2019 Mar 22;10:565. doi: 10.3389/fimmu.2019.00565

Figure 1.

Figure 1

Hypothalamic inflammation following short-term HFD consumption. (A) Confocal images illustrating F4/80+ cells (red) and nuclear labeling with TO-PRO-3® (blue) in the ARC, in coronal brain sections (15-μm) from mice fed with standard chow (SC) or a high-fat diet (HFD) for 3 days; (B) Quantitation of the number of F4/80+ cells in the ARC of mice fed with SC or a HFD (SC, n = 5 and HFD, n = 5); (C) Confocal images illustrating F4/80+ cells (red) and nuclear labeling with TO-PRO-3® (blue) in the ME, in coronal brain sections (15-μm) from mice fed with SC or a HFD for 3 days; (D) Quantitation of the number of F4/80+ cells in the ME of mice fed with SC or a HFD (SC, n = 5 and HFD, n = 5); (E) Confocal images illustrating GFAP+ cells (red) and nuclear labeling with TO-PRO-3® (blue) in the ARC, in coronal brain sections (15-μm) from mice fed with SC or a HFD (3 days); (F) Quantitation of the number of GFAP+ cells in the ARC of mice fed with SC or a HFD (SC, n = 5 and HFD, n = 5). V3, third ventricle; ME, median eminence. The bars represent the mean ± SEM. *Means significantly different as shown by unpaired t-tests (*p < 0.05). Scale bars: 50 μm.