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. 2019 Mar 22;10:565. doi: 10.3389/fimmu.2019.00565

Figure 4.

Figure 4

Cytokine levels in the hypothalamus and peripheral tissues following i.p. LPS challenge and i.p. PNU-282987 stimulation. (A) mRNA expression levels of TNFα (SC, n = 5 and HFD, n = 5); (B) mRNA expression levels of IL-1β (SC, n = 5 and HFD, n = 5); (C) mRNA expression levels of IL-6 (SC, n = 5 and HFD, n = 5); in the hypothalamus; (D) mRNA levels of TNFα (SC, n = 5 and HFD, n = 5); (E) mRNA expression levels of IL-1β (SC, n = 5 and HFD, n = 5); (F) mRNA expression levels of IL-6 (SC, n = 5 and HFD, n = 5) in the spleen; (G) mRNA expression levels of TNFα (SC, n = 5 and HFD, n = 5); (H) mRNA expression levels of IL-1β (SC, n = 5 and HFD, n = 5); (I) mRNA expression levels of IL-6 (SC, n = 5 and HFD, n = 5) in the liver; of mice fed SC or a HFD for 3 days and injected intraperitoneally with a high dose of LPS (12 mg/kg) followed by stimulation with PNU-282987 (3 mg/kg). The bars represent the mean ± S.E.M. *Means significantly different as shown by one-way ANOVA (*p < 0.05; **p < 0.01; #p < 0.05; §p < 0.05). # and § to compare SC and HFD.