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. 2019 Mar 28;9:5303. doi: 10.1038/s41598-019-41740-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Verification of PSC secreted proteins by immunoblot and ELISA. (A) Western blotting of conditioned media concentrates from activated or quiescent PSCs; enolase (ENO-1) was used as control protein. (B) Bar chart of the relative abundance in secretome of non-activated or activated PSCs, respectively, of fibronectin 1 (FN-1) and collagen-1 (Col-1); detection was by commercial ELISA.