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. 2019 Mar 28;9:5303. doi: 10.1038/s41598-019-41740-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The effect of knocking down eIF4E protein expression by small interfering RNA. Tumour cells PT45P1 and Panc-1 were transfected with 100 nM of an eIF4E-specific siRNA or a control siRNA of scrambled sequence for 48 h, followed by serum starvation overnight. Subsequently, they were grown in presence or absence of activated PSC secretome for 24 h. The effect of the various conditions on eIF4E is shown, determined by Western blotting. Also, the results of caspase-3/7 assays are shown for each column (c1 to c6). Note: the two lanes c1 and c2 are exchanged in the Western blots generated from Panc-1 cells.