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. 2019 Mar 28;9:5300. doi: 10.1038/s41598-019-41803-z

Figure 1.

Figure 1

Haematopoietic stem/progenitor cell expansion ex vivo. (a1) Absolute numbers of phenotypically-defined Lin−CD34+CD38−CD45RA−CD90+CD49f+ HSPCs increased significantly with C3-cytokine plus Scriptaid compared to the vehicle control (**p < 0.005; ***p < 0.001. n = 6–9). (a2) Lin−CD34+CD38−CD45RA−CD90− cells decreased in C3-cytokines plus Scriptaid compared to vehicle cultures (**p < 0.005. n = 6–9). (b) Flow plot of 5-day expanded UCB CD133+ cells in vehicle conditions and Scriptaid conditions (right) on the expression of CD45RA against CD90. (c) Estimated number of LTC-IC per well in 5 day Scriptaid vs vehicle control expansion cultures based on LDA results. (n = 6–9, ***p < 0.001). (d) shows CD90− cells becoming CD90+ in the presence of Scriptaid. Cells negative for CD90 (Lin−CD34+CD38−CD45RA−) were sorted and then cultured in C3-cytokine-containing medium supplemented with Scriptaid. Representative flow cytometric analyses are shown. Multiple experiments (n = 3) demonstrate that on average 72 ± 3.1% of MPPs were CD90 positive by two days of culture (***p < 0.001), while CD49f expression levels were only slightly increased (N.S. p = 0.05) on these cultured MPPs. See Supplementary Fig. S2d,e for Median Fluorescence intensity values.