Table 5.

PtNP affects membrane integrity in E.coli and causes leakage of Nucleic acids.

Time (hr)	Untreated		PtNPs (0.02 mM)		Triton X-100 treatment (0.5% V/V)
	Absorbance 260 nm	Absorbance 280 nm	Absorbance 260 nm	Absorbance 280 nm	Absorbance 260 nm	Absorbance 280 nm
1	0.193 ± 0.06	0.130 ± 0.04	0.622 ± 0.08	0.385 ± 0.05	3.047 ± 0.05	2.99 ± 0.11
2	0.248 ± 0.03	0.151 ± 0.04	0.543 ± 0.21	0.251 ± 0.17	3.053 ± 0.15	2.735 ± 0.21
3	0.274 ± 0.01	0.173 ± 0.02	0.690 ± 0.14	0.392 ± 0.07	3.113 ± 0.13	2.820 ± 0.08
4	0.293 ± 0.03	0.228 ± 0.07	0.722 ± 0.01	0.433 ± 0.03	3.137 ± 0.12	2.811 ± 0.11
24	0.48 ± 0.03	0.340 ± 0.02	1.590 ± 0.46	0.889 ± 0.12	3.309 ± 0.08	3.015 ± 0.03