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Anion sepharose exchange chromatography of G. antarctica TRiC. Fractions containing GaTRiC were pooled and concentrated. Figure a) a chromatogram with the arrowhead showing the percentage of MgCl2 needed for TRiC elution and figure b) Immunoblot analysis of fractions eluted from the anion sepharose column using the anti-TRiCα antibodies
