Fig. 8.

Luciferase activity of denatured, native and refolded luciferase incubated with G. antarctica TRiC at different temperatures for 6 h. The activity of native luciferase which was not heat inactivated was 100% while the denatured luciferase was 0%. Renaturation of luciferase was recorded as highest at 4 °C followed by 12 °C, 0 °C, and 30 °C. The percent renaturation were 77%, 71.5%, 9.6%, and 2.5% respectively. Statistical significance was performed using the two-tailed t-test where P < 0.01