Fig. 6.

The JNK signaling pathway participates in mitochondrial stress and cell apoptosis. a and b Caspase-9 activity was measured by ELISA. SP600125 was used to inhibit JNK activation. c and d ATP production was determined in response to JNK inhibition using SP600125. e and f Cell viability was measured via the MTT assay, and SP600125 was used to inhibit JNK activation. g and h The LDH release assay was performed to analyze cell death via ELISA. i–l TUNEL staining for apoptotic cell and the number of apoptosis was recorded in response to SP600125. *p < 0.05 vs. si-ctrl (control siRNA); #p < 0.05 vs. si1-sirt3 (Sirt3 siRNA1)