Fig. 2.

Total antral follicle counts and serum AMH concentrations in aged mares undergoing intra-ovarian injection with MSCs (n = 6) or vehicle (V, n = 2). Follicle numbers were those recorded during videotaping of ultrasound examinations, performed three times weekly with limited exceptions. a Antral follicle counts in individual aged mares for the injected (inj) vs. non-injected (opp) ovaries, relative to date of intra-ovarian injection. Dashed lines represent values for V mares. There were no significant differences in follicle count between ovaries. b Total antral follicle counts for individual aged mares relative to date of intra-ovarian injection. Dashed lines represent values for V mares. There was no significant difference in antral follicle count among the Pre-treatment (8 weeks before injection), Treatment (4 weeks after injection), and Post-treatment (8 weeks after the Treatment period) periods. A line is drawn at the level of five follicles, the minimum required for conducting follicle aspiration. c AMH concentrations in aged mares relative to the date of injection; the same color line represents the same mare as in (b). Samples were taken once weekly. Dashed lines represent values for V mares. Arrowheads: dates when ovariectomies were performed. There were no significant differences in AMH concentration relative to Treatment period