Table 2.
The 20 most differentially expressed genes identified by DESeq2 in MSC1 ovaries compared to vehicle-injected ovaries and in MSC2 ovaries compared to MSC1 ovaries
| Gene symbol | Log2FC | P value | Gene description |
|---|---|---|---|
| MSC1 vs. V | |||
| KRT5 | − 1.55 | 5.70E−05 | keratin 5 |
| KRT14 | − 1.76 | 5.80E−05 | keratin 14 |
| KRT17 | − 1.68 | 2.47E−04 | keratin 17 |
| PRLR | − 1.57 | 1.72E−03 | prolactin receptor |
| TSGA10 | 1.10 | 2.24E−03 | testis specific 10 |
| HIST2H2BE | − 1.21 | 2.53E−03 | histone cluster 2 H2B family member e |
| CNTN5 | 1.40 | 2.55E−03 | contactin 5 |
| CISH | − 1.43 | 3.03E−03 | cytokine inducible SH2 containing protein |
| ASB15 | − 1.48 | 3.42E−03 | Ankyrin repeat and SOCS box containing 15 |
| DCST2 | 1.42 | 4.59E−03 | DC-STAMP domain containing 2 |
| RASSF9 | 1.07 | 4.98E−03 | Ras association domain family member 9 |
| RNF17 | − 1.41 | 5.39E−03 | ring finger protein 17 |
| EPS8L3 | 1.26 | 6.71E−03 | EPS8 like 3 |
| MSLN | 1.37 | 6.76E−03 | mesothelin |
| ERBB4 | 1.34 | 7.10E−03 | erb-b2 receptor tyrosine kinase 4 |
| SLC4A9 | 1.32 | 7.66E−03 | solute carrier family 4 member 9 |
| ADCY1 | − 1.17 | 9.33E−03 | adenyl cyclase 1 |
| TLR1 | 1.09 | 1.14E−02 | toll like receptor 1 |
| IL33 | 1.25 | 1.16E−02 | interleukin 33 |
| ACVR1C | 1.26 | 1.20E−02 | activin A receptor type 1C |
| MSC2 vs. MSC1 | |||
| TAC1 | 4.36 | 7.79E−18 | tachykinin precursor |
| MS4A7 | 1.80 | 4.52E−05 | membrane spanning 4-domains A7 |
| SLAMF9 | 2.01 | 6.82E−05 | SLAM family member 9 |
| PDE6A | 1.84 | 7.89E−05 | phosphodiesterase 6A |
| IRF8 | 1.49 | 3.00E−04 | interferon regulatory factor 8 |
| LGALS4 | − 1.80 | 4.12E−04 | galectin 4 |
| SIGLEC1 | 1.38 | 6.34E−04 | sialic acid binding IG like lectin 1 |
| PLD4 | 1.48 | 6.53E−04 | phospholipase D family member 4 |
| LAT2 | 1.20 | 7.23E−04 | linker for activation of T-cells family member |
| GPR31 | 1.45 | 8.08E−04 | G protein-coupled receptor 31 |
| VAV1 | 1.44 | 8.60E−04 | vav guanine nucleotide exchange factor 1 |
| CD300LB | 1.69 | 8.95E−04 | CD300 molecule like family member b |
| CD163 | 1.52 | 9.80E−04 | CD163 molecule |
| RHBG | 1.63 | 1.08E−03 | Rh family B glycoprotein (gene/pseudogene) |
| GPR151 | 1.22 | 1.19E-03 | G protein-coupled receptor 151 |
| CD86 | 1.47 | 1.23E-03 | CD86 molecule |
| CRADD | −1.05 | 1.30E-03 | CASP2 and RIPK1 domain containing adaptor with death domain |
| SLAMF7 | 1.58 | 1.32E-03 | SLAM family member 7 |
| RASSF9 | −1.13 | 1.40E-03 | Ras association domain family member 9 |
| CYBB | 1.50 | 1.51E-03 | cytochrome b-245 beta chain |
FC, fold change; MSC1, aged mares receiving intra-ovarian injection of MSC on one occasion 14 to 19 weeks before ovariectomy (n = 3); MSC2, aged mares receiving an injection as for MSC1, then a second intra-ovarian injection of MSC 2 weeks before ovariectomy (n = 3); V, aged mares receiving one intra-ovarian injection of vehicle 14 to 19 weeks before ovariectomy