Fig. 4.
Effect of A2AR and H3R activation on cAMP accumulation in rat globus pallidus slices. a Stimulation by the A2AR agonist CGS-21680. Slices were incubated with CGS-21680 for 30 min in Krebs-Henseleit solution (1 mM IBMX). Values are means ± SEM from five experiments. b Inhibition by H3R activation of A2AR-induced cAMP accumulation. Slices were incubated for 30 min with CGS-21680 (10 nM) in the absence or presence of the indicated concentrations of the H3R agonist immepip, added 5 min before CGS-21680. Values for the half-stimulatory (EC50, a) or half-inhibitory concentrations (IC50, b) were obtained by nonlinear regression with GraphPad Prism 5