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. 2019 Mar 22;10:558. doi: 10.3389/fmicb.2019.00558

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Copyright © 2019 Hjelm, Nilvebrant, Nygren, Nilsson and Seijsing.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Schematic sketch of protein complex, (B) structure prediction of complex made using I-TASSER (Zhang, 2008) with mutations highlighted in magenta. (C) Representations of parental lysins and protein constructs designed for this study. The parental endolysin LysK and the derived CHAPK domain is in red. The parental Lysostaphin and its derived SH3b domain are in blue. Barnase and barstar are in green and yellow, respectively. (D). SDS–PAGE of protein constructs. From left to right: ladder, Barnase_E73W, Barnase_E73W-Barnase_E73W, Barstar_C40A/C82A-SH3b, CHAP-Barnase_E73W, CHAP-SH3b, and Barstar_C40A/C82A.