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. 2019 Mar 22;10:558. doi: 10.3389/fmicb.2019.00558

FIGURE 4.

FIGURE 4

Turbidity reduction assays (TRA). Data points were collected every minute and error bars representing standard deviation is shown each 20 min for intelligibility. (A) The CHAP-BarnaseE73W/BarstarC40A/C82A-SH3b complex was used at concentrations of 1000, 333, and 111 nM, respectively (blue dashed/dotted line). The non-complexed controls BarstarC40A/C82A-SH3b (cyan dotted line) and CHAP-BarnaseE73W (orange dashed line) were used at a concentration of 1 μM and PBS (black continuous line) was used as negative control. (B) The CHAP-SH3b gene fusion had a concentration of 14, 4.6, and 1.5 nM, respectively (red dashed/dotted/dotted line) and PBS (black continuous line) was used as negative control.