Table 1.
Melting temperatures, theoretical and experimental molecular weights of constructs.
| Construct name | Theoretical molecular weight (Da) | Experimental molecular weight (Da) | Melting temperature (°C) |
|---|---|---|---|
| CHAP-SH3b | 29 185 | 29 184# | 41.0 |
| CHAP-BarnaseE73W | 32 133 | 32 132# | 42.5 |
| BarstarC40A/C82A-SH3b | 20 933 | 21 292† | 41.0 |
| BarstarC40A/C82A | 12 597 | 12 609† | N/A |
| BarnaseE73W | 13 407 | 13 406# | N/A |
| BarnaseE73W-BarnaseE73W | 26 969 | 26 968# | N/A |
The experimental molecular weights have been determined with MALDI-TOF(†) or LCMS(#). The melting temperature of samples marked N/A were not analyzed.