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. 2019 Mar 28;9:5296. doi: 10.1038/s41598-019-41777-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Reduced smooth muscle layers in cystic airway walls of CPAM. Compared to normal bronchiolar walls, the thickness of the smooth muscle cell layer, detected by MYH11 staining (red), is reduced in CPAM samples. Ciliated epithelial cells were co-stained by anti-TUBB4A antibody (green), and cell nuclei were counterstained with DAPI (blue). Images from 3 randomly selected type 2 CPAM specimens and 3 normal lungs are presented.