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. 2019 Mar 27;21(4):413–427. doi: 10.1016/j.neo.2019.03.003

Figure 6.

Figure 6

LOXL2 promotes breast cancer lymphangiogenesis and lymph node metastasis in vivo. (A) Tumor volume of MCF7-LV and MCF7-LOXL2 mouse models (left). Tumor weight of MCF7-LV and MCF7-LOXL2 mouse models (right). (B) Tumor volume of MDA231-LV and MDA231-shLOXL2 mouse models (left). Tumor weight of MCF7-LV and MCF7-LOXL2 mouse models (right). (C) Representative images of immunofluorescence detection of lymphatic vessels (red, LYVE-1 staining) in MCF7-LV and MCF7-LOXL2 tumors (top). Quantified results of relative lymphatic vessels density were shown (bottom). (D) Representative images of immunofluorescence detection of lymphatic vessels (red, LYVE-1 staining) in MDA231-LV and MDA231-shLOXL2 tumors (top). Quantified results of relative lymphatic vessels density were shown (bottom). (E) Axillary lymph nodes were removed from MCF7-GFP and MCF7-LOXL2 tumor bearing mice. Dissected lymph nodes were photographed (right). Representative images of immunofluorescence detection of GFP-tumor cells in lymph nodes (left). (F) Quantified results of relative metastatic area were shown (left). Sentinel lymph nodes weight of MCF7-LV and MCF7-LOXL2 mouse models (right). (G) Axillary lymph nodes were removed from MDA231-LV and MDA231-shLOXL2 tumor bearing mice. Dissected lymph nodes were photographed (right). Representative images of immunofluorescence detection of GFP-tumor cells in lymph nodes (left). (H) Quantified results of relative metastatic area were shown (left). Sentinel lymph nodes weight of MDA231-LV and MDA231-shLOXL2 mouse models (right). The data are the means ± SD of three independent experiments. n = 6. *P < .05; **P < .01; ***P < .001. Scale bar = 100 μm.