LOXL2 activates CAFs in breast cancer in vivo. (A) Representative images of immunofluorescence detection of CAFs (red, α-SMA staining) in MCF7-LV and MCF7-LOXL2 tumors (top). Quantified results of relative α-SMA positive regions were shown (bottom). (B) Representative images of immunofluorescence detection of CAFs (red, α-SMA staining) in MDA231-LV and MDA231-shLOXL2 tumors (top). Quantified results of relative α-SMA positive regions were shown (bottom). (C, D) The mRNA levels of SDF-1α and VEGF-C in fibroblast cells isolated from tumor tissues. n = 6, error bars represent SD. P values, Student's t test, **P < .01; ***P < .001. Scale bar = 100 μm.