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. 2019 Mar 22;13:56. doi: 10.3389/fnbeh.2019.00056

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Copyright © 2019 Asok, Hijazi, Harvey, Kosmidis, Kandel and Rayman.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Remote contextual fear generalization using context that retains metal grid floor used in the training context. (A) Experimental design. Context A and Context C are completely different in terms of odor, chamber shape, and lighting. However, the same metal grid floor through which shocks were delivered during training is present in both contexts. (B) Freezing behavior of male and female mice in remote contextual generalization, with both test orders (A→C and C→A). Significant Bonferroni post hoc effects following three-way ANOVA are indicated. (C) Discrimination index calculated from freezing data, with Bonferroni post hoc test following two-way ANOVA revealed a significant effect of test order in females. ***p < 0.001 and ^#p < 0.0001. Error bars are mean ± SEM.