Figure 3.

Endogenous in vivo model for studying metabolite aggregation. A novel yeast-based model for in vivo study of metabolite aggregation phenomena was recently established (Laor et al., 2019). In contrast to the classical yeast models of amyloid-associated diseases, this system does not rely on exogenous expression, but rather on the endogenous disruption of a gene-of-interest, thereby leading to the accumulation, and consequential aggregation of a disease-associated metabolite. Thus, the adenine salvage model, which recapitulates a rare inborn error of metabolism disorder, exhibits similar characteristics to other yeast amyloid models, including a prominent growth phenotype, staining with amyloid-specific dye, etc. Scale bar is 5 μm.