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Analytical HPLC of purified mSAM. Gradient of 50–100% acetonitrile. RT: 10.7 min (lower panel), 11.5 min (upper panel). Absorbances at 450 nm (coumarin 343), 540 nm (TAMRA), 254 nm, and 280 nm are shown. Both 5-(6-)TAMRA isomers are shown separately (upper and lower panel).
