Figure 6.
Analytical HPLC of purified sSAM. Gradient of 10–100% acetonitrile, RT: 9 and 9.5 min. Absorbance at 450 nm (coumarin 343), 540 nm (TAMRA), 254 nm, and 280 nm are shown. The two peaks represent the two 5-(6-)TAMRA isomers.
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