In vivo topical application of PCN-miR/Col reshapes
the highly oxidative and inflammatory wound microenvironment and corrects
miR-26a overexpression. Representative confocal images of immunofluorescence
staining and quantification for (A) 8-OHdG (a marker of oxidative
DNA damage), (B) 4-HNE (a marker of lipid peroxidation), (C) CD68-positive
macrophages, (D) miR-26a, and (E) Ki67-positive cells in sections
from each group after 28 days of treatment (n = 4).
Scale bars for 8-OHdG, 4-HNE, CD68, and Ki67 images, 50 μm;
Scale bars for miR-26a image, 100 μm. All results are presented
as mean ± SD, *P < 0.05 by two-tailed unpaired
Student’s t tests.