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Five times the Km amount of the substrates: (A) O6BG, (B) DACA, (C) zaleplon, (D) phthalazine (E) BIBX1382, and (F) zoniporide were incubated with HLC (0.0051 μM AO) at 37°C, and samples were quenched at different time points over 240 minutes (n = 3, P < 0.001). Data fitting was performed using the MAM. Based on the results, unlike the MM assumption, enzyme activity is not linear over time.
