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Catalase and SOD (250 U/ml) were used to remove ROS that were suspected to affect the enzymatic activity. HLC (0.0051 M AO) was used to start the reactions with A) O6BG, B) DACA, C) zaleplon, D) phthalazine E) BIBX1382, and F) zoniporide, (n = 3, P < 0.001). No significant change in linearity of the enzyme was observed once these reagents were used, suggesting that ROS are not the reason behind the decrease in enzymatic efficacy.
