Skip to main content
. 2019 Feb 27;54(5):1691–1703. doi: 10.3892/ijo.2019.4733

Figure 4.

Figure 4

ZNF692 overexpression promotes the growth, migration and invasion of colon adenocarcinoma cells. HCT116 cells were transfected with either the pcDNA-ZNF692 overexpressing vector or an empty vector control. (A) RT-qPCR and (B) western blot analysis of the transfection efficiency of pcDNA-ZNF692 in HCT116 cells. (C) Proliferation and (D) colony formation capability were estimated by CCK-8 and colony formation assays, respectively. (E) Flow cytometry showed the distribution of pcDNA-ZNF692-transfected HCT116 cells in the G1, S, and G2/M phases of the cell cycle. (F) Cell migration ability was assessed by wound healing and (G) Transwell migration assays. (H) Invasion was assessed by the Transwell invasion assay in pcDNA-ZNF692-transfected HCT116 cells and control cells. Magnification, ×100. Scale bar, 100 µm. The experiments were performed three times, and the data are presented as the mean ± standard deviation. *P<0.05 and **P<0.01 vs NC. ZNF692, zinc finger protein 692; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; CCK-8, Cell Counting Kit-8; NC, negative control; OD, optical density.