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. 2019 Feb 27;54(5):1691–1703. doi: 10.3892/ijo.2019.4733

Figure 6.

Figure 6

ZNF692 is involved in regulating the PI3K/AKT signaling pathway. (A) Representative images and (B) quantification from western blot analysis of the effects of ZNF692 knockdown on the PI3K/AKT pathway and the downstream factors cyclin D1, CDK2, p27 and MMP-9 in DLD-1 and LoVo cells. (C) Representative images and (D) quantification from western blot analysis of the effects of ZNF692 overexpression on the PI3K/AKT pathway and the downstream transcription factors cyclin D1, CDK2, p27 and MMP-9 in HCT116 cells. (E) Western blot analysis demonstrated that LY294002 effectively decreased the expression levels of cyclin D1 and MMP-9 induced by ZNF692 overexpression. The experiments were performed three times, and the data are presented as the mean ± standard deviation. *P<0.05 and **P<0.01 with comparisons indicated by brackets. ZNF692, zinc finger protein 692; PI3K; phosphoinositide 3-kinase, AKT, AKT serine/threonine kinase; CDK, cyclin-dependent kinase; MMP, matrix metalloproteinase; p-, phosphorylated; sh, short hairpin; NC, negative control.