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. 2019 Mar 29;19:116. doi: 10.1186/s12870-019-1688-z

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — The interaction of GhWRKY27 with GhTT2, GhBTF3 and GhRD21A was determined in yeast cells and Arabidopsis mesophyll protoplasts. a Physical interaction of GhTT2, GhBTF3 and GhRD21A with GhWRKY27 in yeast cells. pGBKT7-p53 + pGADT7-largeT and pGBKT7-laminC + pGADT7-largeT were used as positive and negative controls, respectively. b The interaction of GhTT2, GhBTF3 and GhRD21A with GhWRKY27 in Arabidopsis mesophyll protoplasts. The yellow signal indicates YFP fluorescence, and the red signal indicates the autofluorescence of chlorophyll. The merged image is a combination of images showing the YFP and chlorophyll signals. No YFP signals were detected in the negative controls