Fig. 3.

miR-96-5p expression affects radiosensitivity of Cal 27 and FaDu cells. Cal 27 and FaDu cells were transfected with miR-96-5p mimic and inhibitor. After 48 h, cells were treated with 2Gy dose of irradiation. a-d RT-qPCR analysis showing efficiency of miR-96-5p mimic transfection in Cal 27 cells (a) and in FaDu cells (c). Cell viability after transfection and radiation of Cal 27 (b) and FaDu (c) cells has been assessed by ATPlite assay. e-h RT-qPCR analysis showing efficiency of miR-96-5p inhibitor transfection in Cal 27 cells (e) and in FaDu (g) cells. Cell viability after transfection and radiation of Cal 27 (f) and FaDu (h) cells has been assessed by ATPlite assay. i-j Colony formation assay of Cal 27 (i) and FaDu (j) cells after transfection with miR-96-5p inhibitor and radiation treatment at 2Gy dose. Histogram bars show the means of at least three experiments performed in triplicate. *P < 0.05; **P < 0.001. NC: Negative control