Fig. 4.

miR-96-5p expression affects the sensitivity of Cal 27 cells to chemotherapy. Cal 27 cells were transfected with miR-96-5p mimic and inhibitor. After 48 h cells were treated with different concentration of cisplatin (0.5,1,2,4,8 μg/ml). a RT-qPCR data showing efficiency of miR-96-5p mimic transfection. b Cell viability analysis of Cal 27 cells treated with miR-96-5p mimic and different concentrations of cisplatin in comparison with the negative control. c RT-qPCR data showing efficiency of miR-96-5p inhibitor transfection. d Cell viability analysis of Cal 27 cells treated with miR-96-5p inhibitor and different concentration of cisplatin in comparison with the negative control. e Colony formation assay performed on Cal 27 cells treated with miR-96-5p inhibitor and different concentration of cisplatin in comparison with the negative control. Histogram bars show the means of at least three experiments performed in triplicate.*P < 0.05; **P < 0.001. NC: Negative control, CDDP: Cisplatin