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. 2019 Mar 29;38:141. doi: 10.1186/s13046-019-1119-x

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — PTEN silencing mimics the biological effects of miR-96-5p overexpression in Cal 27 cells. a Western-blot analysis of PTEN protein expression level in Cal 27 cells transfected with siPTEN and scrambled siRNA as negative control. b Representative histogram of transwell migration assay of Cal 27 cells treated with siPTEN and scrambled siRNA and co-transfected cells with miR-96-5p inhibitor and siPTEN or control inhibitor and scrambeld siRNA. Bars show the means of at least two experiments performed in triplicate. The p-value refers to matched control inhibitor and scrambled siRNA versus miR-96-5p inhibitor and siPTEN transfected samples.*P < 0.05; **P < 0.001; ***P < 0.0001. c Colony formation assay of Cal 27 cells treated with siPTEN and scrambled siRNA. Histogram shows the number of migrated cells after using siPTEN and scrambled siRNA. Bars show the means of at least two experiments performed in triplicate. The p-value refers to matched control versus siPTEN transfected samples.*P < 0.05; **P < 0.001; ***P < 0.001. d-e Cell viability (d) and cell colony (e) analyses of Cal 27 cells transfected with siPTEN and treated with radiation at the dose of 2Gy. f Cell viability analysis of Cal 27 cells transfected with siPTEN and treated with different concentration of cisplatin in comparison with the negative control. siSCR: SCR siRNA, siPTEN: PTEN siRNA