Table 2.

Run time and cost estimates

Sample size	Per-sample preprocessing (Hours/$)	Aggregation and QC (Hours/$)	Total (Hours/$)
10	0.98 ($0.93)	0.97 ($0.28)	1.95 ($1.21)
100	1.47 ($8.99)	6.00 ($0.86)	7.47 ($9.85)
1000	4.48 ($52.48)	58.01 ($13.74)	62.49 ($66.22)

Time and cost to conduct various steps of the workflow with different sample sizes. Estimates were obtained when the workflows were run on the default n1-highmem-4 compute nodes (26 GB RAM with 4 CPUs) in FireCloud. Note that these example times and costs will decrease considerably as workflows are improved and compute resources become cheaper