Table 1.
Equilibrium dissociation constants (KD), association rate constants (ka), and dissociation rate constants (kd) for the amyloid β (Aβ)-binding Affibody molecules, determined by KinExA.
| Molecule | Measured interaction | KD (pM, the best KinExA fit and 95% CIa) | ka (M−1s−1, the best KinExA fit and 95% CIa) | kd (s−1) |
|---|---|---|---|---|
| ZSYM73-His6 | Aβ40 | 12.1 (9.02–15.9) | 7.45 (6.97–7.93) × 105 | 9.01 × 10−6 |
| Z SYM73-His6 | Aβ42 | 21.3 (15.6–28.4) | 9.99 (8.29–11.8) × 105 | 2.13 × 10−5 |
| Z SYM73-ABD | Aβ40 | 68.6 (39.8–106) | 4.50 (4.25–4.74) × 105 | 3.07 × 10−5 |
| Z SYM73-ABD | Aβ42 | 61.4 (40.1–88) | 1.92 (1.8–2.04) × 106 | 1.18 × 10−4 |
| ZSYM73-ABD | HSA | 53.7 (20–98.2) | 5.08 (4.58–6.62) × 105 | 2.73 × 10−5 |
aThe 95% confidence interval of the KinExA fit is shown in parentheses.