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. 2019 Mar 22;10:538. doi: 10.3389/fimmu.2019.00538

Figure 1.

Figure 1

Levels of C3d in spleens and kidneys from WT, MMP-9−/−, LPR−/−, LPR−/−/MMP-9−/− mice. (A,B) Immunohistochemical analysis of C3d as read-out of classical IC-mediated complement activation in WT, MMP-9−/−, LPR−/−, and LPR−/−/MMP-9−/− spleens (A) and kidneys (B). The horizontal bars in the images represent 50 μm for a magnification of 20 times for spleens and 10 μm for a magnification of 40 times for kidneys. Brown staining represents semi-quantitatively the level of complement factor C3d. (C,D) Anti C3d ELISAs of spleen tissue extracts (C) from 9 WT, 14 MMP-9−/−, 16 LPR−/− and 17 LPR−/−/MMP-9−/− mice and of kidney tissue extracts (D) of 13 WT, 16 MMP-9−/−, 16 LPR−/−, and 17 LPR−/−/MMP-9−/− mice. P-values were determined by ANOVA Kruskal-Wallis test. *p < 0.05, **p < 0.01, and ***p < 0.001. Throughout the manuscript the following color code was used: yellow=WT, blue-=MMP-9−/−, red=LPR−/− and green=LPR−/−/MMP-9−/−.