Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Mar 22;7:42. doi: 10.3389/fcell.2019.00042

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2019 Cao, Liccardo, LaCanna, Zhang, Lu, Finck, Leigh, Chen, Drosatos and Tian.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Cardiomyocyte proliferation with etomoxir (ETO) treatment. (A) Cardiomyocytes in DNA synthesis-phase were detected by using Click-iT EdU Alexa Fluor (red) and co-immunostaining with antibody against cTnT (green) on tissue cross sections. Quantification of EdU+cTnT+ cells as percentage of total cTnT+ cells analyzed per field. Arrows point to EdU+cTnT+ cells. (B) Cardiomyocytes in mitotic phase were detected and quantified by immunostaining for PH3 (red) and cTnT (green) on tissue longitudinal sections. Arrows point to PH3+cTnT+ cells. (C) Cardiomyocyte proliferation were visualized by co-immunostaining of heart sections (P5) for Ki67 (red) and cTnT (green). Graph on the right showing quantification of Ki67+cTnT+ as percentage of total cTnT+ cells analyzed per field. Arrows point to Ki67+cTnT+ cells. (D) Percentage of mononuclear (Mono-CM) and binuclear (Bi-CM) cardiomyocytes in the heart ventricles of infant mice at P5. (E) Total number of cardiomyocytes in heart ventricles in infant mice at P5. (F) Immunostaining and quantification of TUNEL (green) and DAPI (blue) in P5 heart sections. Arrows point to TUNEL+ nuclei. (G) Expression of Bax and Bcl2 by qRT-PCR analysis of the mRNA of isolated heart ventricles at P5 (n = 4 per group). P value was calculated using Student’s t-test (A–C, E–G) and two-way ANOVA (D).