Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Mar 22;7:42. doi: 10.3389/fcell.2019.00042

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2019 Cao, Liccardo, LaCanna, Zhang, Lu, Finck, Leigh, Chen, Drosatos and Tian.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Cardiomyocyte proliferation and hypertrophic growth in GW7647-treated infant mouse hearts. (A) Infant mice were treated either with GW7647 or saline at P2, P3 and P4. Quantification of genes associated with fatty acid metabolism by qRT-PCR analysis of the mRNA of isolated heart ventricles at P5 (n = 4 per group). (B) Quantification of EdU+cTnT+ cells as percentage of total cTnT+ cells isolated from heart ventricles at indicated time points (n = 6 per group, ∼1000 cTnT+ cells per sample). (C) Quantification of total number of cardiomyocyte isolated from heart ventricles at indicated time points (n = 4–8 per group). (D) Representative images of isolated cardiomyocytes from P5 heart ventricles and quantification of the percentage of mononucleated (Mono-CM) and binucleated (Bi-CM) cardiomyocytes (n = 4–6 per group, ∼1000 cTnT+ cells per sample). Arrows point to Bi-CM. cTnT (blue). DAPI (gray). (E,F) Ventricular cardiomyocytes were isolated at P5 and measured for surface area. Quantitative analyses represent frequency distribution (E) and mean square areas (F) of the surface area of cardiomyocytes. (G) Quantification of genes associated with cardiomyocyte maturation by qRT-PCR analysis of the mRNA of isolated heart ventricles at P5 (n = 4–5 per group). P value was calculated using Student’s t-test (A, F,G), one-way ANOVA (D) and two-way ANOVA (B,C).