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. Author manuscript; available in PMC: 2019 Mar 29.
Published in final edited form as: Anal Chem. 2018 Dec 3;90(24):14294–14302. doi: 10.1021/acs.analchem.8b03520

Figure 1.

Figure 1.

Workflows of intact glycopeptide analysis strategies for lysate and medium from human EPO-expressing CHO-K1 cells. (a) The workflow of large scale deglycosylated peptide analysis enriched by MAX extraction cartridges. The proteins were digested, fractionated and MAX enriched. The glycopeptides were then deglycosylated and analyzed by LC-MS/MS. (b) Different glycopeptide analysis strategies using MAX cartridge enrichment followed by fractionation or fractionation of global peptides, followed by enrichment of IGPs using MAX cartridges for large-scale IGPs analysis.