Figure 2.

Depth of the identified IGPs in cell lysate and medium from human EPO-expressing CHO-K1 cells. (a) Identification and distribution of total and subtype IGPs identified from cells and medium. (b) Distribution of identified glycosites and glycoproteins in cell lysate and medium, showing that most glycoproteins are present in CHO-K1 cell lysate and medium. (c) Identification and distribution of glycans and the composition and distribution of fucosylated and sialylated N-linked glycans in cell lysate and medium.