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. Author manuscript; available in PMC: 2020 Mar 11.
Published in final edited form as: Dev Cell. 2019 Feb 21;48(5):617–630.e3. doi: 10.1016/j.devcel.2019.01.021

Figure 6. Phagocytic role of EcTMs.

Figure 6.

A. Volcano plots of RNA-seq from EcTMs and non-EcTMs. 36 and 4 genes are expressed at significantly higher and lower level in EcTMs, respectively. n=4, each

B. Clustered heatmap of the genes enriched in EcTMs. n=4, each

C. Gene ontology analysis of the genes enriched in EcTMs.

D. Macrophage phagocytosis assay. EcTMs and non-EcTMs are sorted from P8 hearts and incubated with FITC-labeled latex beads. The fraction of phagocytic macrophages were higher in EcTMs. Data represent mean ± SE. n=5. *P < 0.05 by paired t-test.

E-J. Hematoxylin and Eosin (H-E) staining (E, H) and electron microscopy images (F, G, I, J) of the endocardial cushion at E13.5. Magnification is 4800x (F), 7200x (G), 3600x (I), and 7200x (J) magnification. Note the large cells (white arrows) with phagosomes containing apoptotic cells (black arrows) and particles (black arrowhead). Scale bar = 2.3 um