Fig. 2A-G. NET specificity of D22 analogs by displacement of [³H]Nisoxetine binding in mouse hippocampus.

Compounds 1–7 (A-G, black, open circles) were tested for their ability to displace the high-affinity NET blocker [³H]nisoxetine in 120 min binding assays performed on ice. Concentration-response curves obtained for unlabeled reference compounds are repeated in gray for each subfigure and include NET substrate dopamine (gray, closed squares), inhibitor desipramine (gray, closed circle), and decynium-22 (D22, gray, open square). The IC₅₀ determinations obtained from non-linear regression curve fits can be found in Table 2.