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. Author manuscript; available in PMC: 2020 Jan 5.
Published in final edited form as: Eur J Pharmacol. 2018 Oct 25;842:351–364. doi: 10.1016/j.ejphar.2018.10.028

Fig. 4A-C. High-affinity/low-capacity transporter competitive uptake inhibition by cocaine, corticosterone, and decynium-22.

Fig. 4A-C.

Inhibition of [3H]MPP+ uptake was measured in HEK cells transiently transfected with mouse DAT (A), NET (B) for 3 min, or inhibition of [3H]5-HT uptake measured in SERT-HEK cells (C) for 1 min. Concentrations of unlabeled cocaine (Coc, ●), corticosterone (CORT, ■), and decynium-22 (D22, □) ranged from 0 nM to 1 mM. Concentration-response curves were fit to a non-linear regression analysis and IC50 estimations determined as follows in μM [95% CI]: DAT (A) Coc = 0.18 [0.09–0.32], CORT = 364.6 [154.7–859.4], D22 = 12.9 [9.8–16.9]; NET (B) Coc = 1.2 [0.8–1.8], CORT = 840 [334–2113], D22 = 35.0 [25.0–49.2]; and SERT (C) Coc = 0.51 [0.22–1.2], CORT = 444.7 [128.6–153.8], D22 = 7.9 [6.4–9.7]. Data represents an n of 3 independent experiments performed in duplicate fit to a non-linear regression analysis to determine estimated IC50 values (refer to Table 3).